Custom Adenovirus Production Full Service, Pilot Scale


  • Catalog #: SL100736
  • Unit Size: 1.0 mL at >5E+10~5E+11 PFU/mL)

Custom Adenovirus Production Full Service, Pilot Scale

Production of Recombinant Serotype 5 Adenovirus (E1/E3 deletion) with Ad.MAX System:
- Unsurpassable transduction efficiency (nearly 100% vs. AAV 30~40% and Lentivirus ~30%) in most mammalian cells.
- No integration into host genome.
- Compatible with toxic gene of interest (GOI).
- Induce high-level transient protein expression.
- Accommodate inserts of up to 7.5 kb.


Large collection of Ad.MAX™ shuttle vector for cloning shuttle plasmid with a specific promoter and reporter:

Single promoter Ad.MAX™ shuttle vector

Dual promoter Ad.MAX™ shuttle vector

              Promoterless
              CMV
              CAG
              H1
             
U6
              Synapsin
              UBC

              EF1α
              ALB(1.4)
              ApoE/AAT1
              CaMKII
              ELA1
              Enh358MCK
              cTNT
              GFAP
              MBP
              SST
              TBG

              αMHC
              hRPE
              mIP1
              tMCK



















 

               Promoterless
               CMV
               CAG
               H1
              
U6
               Synapsin
               UBC

               EF1α
               ALB(1.4)
               ApoE/AAT1
               CaMKII
               ELA1
               Enh358MCK
               cTNT
               GFAP
               MBP
               SST
               TBG

               αMHC
               hRPE
               mIP1
               tMCK

Promoterless
CMV
CAG
H1

U6
Synapsin
UBC

EF1α
CaMKII
cTNT
GFAP










 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


Service Description:
1. Clone your gene of interest (GOI) into a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Initial adenoviral stock production.
3. Adenovirus amplification from
~500 cm2 Ad.MAX™ 293 cells.
4. Recombinant adenovirus PFU titration.

Required Material: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).

Turnaround Time: 3 ~ 4 weeks.

Deliverables: >1.0 mL (5x0.2 mL) super infectious in vitro grade of adenovirus vector at 5E+10~5E+11 PFU/mL*.

We offer discount for new customers, please request a quote
with us today.

We offer truncated service as well, please request a quote with us today.


* Final adenovirus yield may depend on the nature of transgene.



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